Born and raised in Norfolk, Virginia, Gustin always enjoyed performing. While attending Granby High School, he attended the prestigious Governor’s School for the Arts program to focus on musical theatre and appeared in Cabaret, All Shook Up, and Little Shop of Horrors. After graduation, Gustin was admitted into the BFA Music Theatre Program at Elon University in North Carolina. It was during his sophomore year that he decided to audition for the Broadway revival tour of West Side Story and was awarded the role of Baby John and toured across the country with over 400 performances.
After wrapping the tour, Gustin joined the third season of the popular musical series, Glee in which he played Sebastian Smythe, the lead of Dalton Academy's Warblers singing group.
Other television credits include playing twins on CSI: MIAMI a recurring role on the long-running teen drama, 90210; and a lead in the original Lifetime movie, A Mother’s Nightmare, opposite actress Annabeth Gish.
On the film side, Gustin was part of the ensemble cast of Affluenza, directed by Kevin Asch. The film is a generational coming-of-age drama set amid an upper class Long Island suburb in the final weeks of the summer leading up to the financial meltdown of 2008.
Gustin has made several crossover appearances on Arrow and continues his role as Barry Allen aka The Flash.
Gustin was recently awarded the inaugural Artist Breakthrough Award at the 2015 Saturn Awards. He was also voted by fans as their Breakout Star for the 2015 Teen Choice Awards.